Ch 9-13 SG Audesirk

Ch 9 -
    Complete the Thinking through the Concepts questions #1-6 on page 165 of your text.

Ch 10 SG-

   
     #1-5 are worth 2 pts each. #6 is worth 10 pts.
1.     How does RNA differ from DNA?

2.     What are three types of RNA? What is the function of each?

3.      Define mutation. Give one example of how a mutation might occur.

4.     The base sequence of the gene coding for a short polypeptide is CTACGTAGGCGATTACTA.
        What would be the base sequence of mRNA transcribed by this gene?
        Using the genetic code chart (p172 Audesirk), give the amino acid sequence of the polypeptide translated from this mRNA
5.      Some antibiotics such as erythromyacin and streptomyacin bind to the small robosomal subunit and inhibit translation. Why do these drugs kill bacteria? How can                  bacteria evolve so that they become resistant to such antibiotics?

6.     Select terms from the list provided to compare DNA replication, transcription and translation.(10 pts)

+1 (transcription initiation site)                 origin of replication
3'--> 5'                                                         N terminal to C terminal
AUG (start codon)                                      promoter
5'-->3'                                                          polypeptide
amino acids                                                 RNA
aminoacyl-tRNA synthase                         ribonucletides
deoxribonucleotides (A,C,T,G)                  RNA polymerase           
DNA                                                             ribosome           
DNA Polymerase                                        ribosome binding site         
mRNA                                                          stop condon          
                                                                      terminator          
  

 
DNA replication
Transcription 
Translation 
Macromolecule 
synthesized
. . .
Monomers
polymerized
. . .
What molecule is used
as the template ?
. . .
 In what direction is the new
molecule synthesized?
. . .
What enzyme or molecule
brings about the 
polymerization reaction?
. . .
The site on the template molecule
where the RNA polymerase
or ribosome binds is called?
. . .
The site on the template molecule
where synthesis begins is called?
. . .
The site on the template molecule
where synthesis ends is called?
. . .

   

Ch 11 SG-
 (review online at  http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/cell_division.html     )

Answer the following questions in complete sentences on your own paper. (3.3 points each)

1. In what ways are mitosis and meiosis similar. In what ways are they different?

2. Both nerve cells in the adult human nervous system and heart muscle cells  remain in the G portion of interphase. In contrast, cells lining of the small intestine divide frequently. Discuss this difference in terms of why strokes and heart attacks are so dangerous. What would happen to tissues in the small intestine if some disorder or a drug blocked mitosis in all cells of the body?

3. Cancer cells divide out of control. Chemotherapy and radiation therapy typically cause the loss of hair and the lining of the intestinal tract, producing severe nausea. What can you infer from this about the mechanism of these treatments?

4. Vinblastine and taxol (which is extracted from the bark of the Pacific yew) are both drugs which interfere with the assembly of microtubules.How would these drugs function to treat cancer?

5. Many species can reproduce either sexually or asexually, It is often when the environment changes in some way that is unfavorable to an existing population that the organisms begin to reproduce sexually. Discuss the advantages and disadvantages this behavior might have on the evolutionary survival of the species  or on the survival of individuals.

BONUS:  (Give this a try- it really isn't that hard)When a population of cells is examined with a microscope, the percentage of cells in the M phase is called the mitotic index. The greater the proportion of cells dividing, the higher the mitotic index. In a particular study, cells from a cell culture are spread on a slide, preserved and stained, then inspected with a microscope.  A hundred cells are examined: 9 cells are in prophase; 2 cells are in anaphase; 4 cells are in telophase; 5 cells ar in metaphase; 2 cells in anaphase; 4 cells in telophase; the remainder, 80 cells, are in interphase. Based on this information, answer the following questions:

a. what is the mitotic index for this cell culture?
b. The average duration for the cell cycle in this culture in known to be 20 hours. What is the duration of interphase? Metaphase?
c. Going back to the living cultures of cells, the average quantity of DNA is measured. Of the cells in interphase, 50% contain 10 ng ( 1 nanogram = 10-9g) of DNA per cell, 20% contain 20ng of DNA per cell and the remaining 30% of the interphase cells have amounts of DNA between 10 and 20 ng. Based of this data, determine the average length of the G1, S and G2 portions of the cell cycle.


Ch 12 SG-

Be sure you can solve the gentics problems shown on pg 238 in your text.

For the questions below, in all cases show pedigree charts OR punnet squares to justify your answer.

         1.   A man has six fingers on each hand and six toes on each foot. His wife and their daughter have the normal number of digits. Extra digits is a dominant trait. What fraction of this couple's children would be expected to have extra digits.
       

2.    Bill and his wife Marie hope to have children. Both have notably flat feet and long eyelashes and they tend to sneeze a lot( hence the name of the  achoo syndrome)A dominant allele gives rise to each of these traits: A (foot arch), E (eyelash length), S (chronic sneezing). Bill is heterozygous of all three traits and marie is homozygous for all three traits.

                         a. What is Marie's phenotype? What is Bill's?
                         b. Marie becomes pregnant three times. What is the probability that each child will show all three of Bill's traits? Of Marie's?
                         c. What is the probability that each child will have short lashes, high arches and no chronic tendency to sneeze?


3.    Imagine you are a genetic counselor and a couple planning to start a family came to you for information. Charles was married once before and his first wife had a child which has cystic fibrosis. The brother of his current wife, Elaine, died of cystic fibrosis. What is the probability that Charles and Elaine will have a baby with cystic fibrosis? (Neither Charles nor Elaine has cystic fibrosis).


         
4.   In mice, black coat color (B) is dominant to white(b). At a different locus, a dominant allele (A)produces a band of yellow just below the tip of  each hair in mice with black fur. This gives the frosted appearance known as agouti. Expression of the recessive allele(a) results in a solid coat color. If mice that are heterozygous at both loci are crossed, what will be the expected phenotypic ration of their offspring.


        5.  Kernel color in wheat plants is determined by two pairs of genes. Alleles of one pair show incomplete dominance over alleles of the other pair. For  the one gene pair at one locus on the chromosome, allele A1 imparts one dose of red color to the kernel whereas Adoes not. A second allele B1 gives one dose of the red color to the kernel while Bdoes not. A kernel with genotype A1A1B1B1 is dark red. A kernel with genotype  A2A2B2Bis white.  All other genotypes have kernel colors between these extremes.


                      a. If you cross a plant grown from a dark red kernel plant and one with a white kernel plant what genotypes and phenotypes would be expected among the offspring? In which proportions?


                      b. If a plant A1A2B1B2 self fertilizes, what are the expected genotype and phenotype ratios.


         7. You could not have gotten a sex chromosome from one of your four grandparents. Which grandparent could not have transmitted (via your parents) a sex chromosome to you . (Note your answer will depend upon your sex).

BONUS: Although they are rare, male claico cats do occur. In fact, about 1 in 3000 calico cats is male. Most male calico cats are infertile. Explain what causes the calico trait in males   and why most would be infertile.

Ch 13 SG-  (25 points)

Answer the Thinking through the Concepts in your text # 1-7 ( 2 pts each)

8.
A game warden in Africa confiscated eight ivory tusks from elephants. Some tissue is still attached from the tusks. Now he most determine if the tusks were taken illegally from northern populations of endangered elephants or from other elephants from populations to the south which can be hunted legally. How can he use DNA fingerprinting to find out the answer?  (6 pts)


Jurassic Park questions are worth 50 points: